Background. Chronic periodontitis is a multifactorial infectious disease, where multiple bacteria, such as Porphyromonas gingivalis,\nPrevotella intermedia, and Fusobacterium nucleatum are implicated. The main purpose of researching natural products is\nto find substances or compounds with antimicrobial activity. Aim. The objective of this work was to determine antimicrobial\nactivity from extracts and obtained fractions from Piper marginatum Jacq and Ilex guayusa Loes on P. gingivalis ATCC 33277, F.\nnucleatum ATCC 25586, and P. intermedia ATCC 25611. Methods. Total ethanol extracts were obtained from both plants.\nFractions were obtained from total ethanol extracts with amberlite as a stationary phase employing hexane, acetone, and ethanolwater\nas solvents. Qualitative and quantitative phytochemical characterization was performed on total ethanol extracts from both\nplants. Antimicrobial activity from total ethanol extracts and fractions from both plants were evaluated on P. gingivalis ATCC\n33277, F. nucleatum ATCC 25586, and P. intermedia ATCC by the well diffusion method with Wilkinsâ??Chalgren agar. Results.\nPiper marginatum Jacq total ethanol extract presented antimicrobial activity against all three bacteria, whereas Ilex guayusa Loes\nwas only efficient against P. gingivalis ATCC 33277 and P. intermedia ATCC 25611, with inhibition halos from 9.3 to 30 mm. Ilex\nguayusa Loes obtained fractions presented antimicrobial activity against all three microorganisms evaluated, with inhibition halos\nranging from 9.7 to 18.7 mm. In regards to Piper marginatum Jacq fractions, inhibition halos were between 8.3 and 19 mm, against\nall three microorganisms evaluated; only hexane fraction did not present antimicrobial activity against F. nucleatum ATCC 25586.\nConclusion. Piper marginatum Jacq and Ilex guayusa Loes total ethanol extracts and fractions presented outstanding antimicrobial\nactivity against P. gingivalis ATCC 33277, P. intermedia ATCC 25611, and F. nucleatum ATCC 25586.
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